World Class Filtration Solutions
Chromatin immunoprecipitation (ChIP) is a technique used to study the association of specific proteins, or their modified isoforms, with defined genomic regions. In a ChIP assay, DNA-protein complexes (Chromatin) are identified using matching antibodies and the resulting fractions treated to separate the DNA and protein components. Polymerase Chain Reaction (PCR), Real Time PCR, hybridization on microarrays, or direct sequencing are typically used to identify DNA fragments of defined sequence.
The traditional ChIP methods, based on agarose or magnetic beads, involve many steps of separation, pipetting and re-suspension. These sequences can either be done by magnetism or centrifugation, which are repetitive and prone to errors.
Chromatrap® is a new technology that offers a quicker, easier and more efficient way of undertaking chromatin immunoprecipitation assays. These kits use spin columns which contain discs of an inert, porous polymer to which protein A or protein G has been covalently attached. During an assay the target chromatin/antibody complex is retained by the disc. Flushing with three buffers and an elution step are all that is required to obtain the DNA fragments of interest.
Please visit www.chromatrap.com for more information or details on how to contact one of our Chromatrap® representatives.